Cinnah Cad. No:54 Çankaya Ankara Türkiye

TIME-LAPSE SYSTEM FOR OBSERVATION OF EMBRYO DEVELOPMENT

Don't you want to follow all stages of your embryo development?
 
Embryos are followed more closely by time-lapse incubators.

 
Eggs/embryos are kept (cultured) in special devices called incubators in an IVF laboratory.  Following microinjection of an egg with an oocyte, the microinjected oocyte is transferred in a dish to an incubator.  The morphological development of an embryo (cell number, fragmentation etc.), thereafter, is evaluated under microscopy at universally set time-points by taking the embryo out of the incubator.  However, taking the embryo each time out of the incubator poses a stress to the embryo due to disturbed culture conditions; the ambient temperature in a classical lab is 23oC versus 37 oC in the incubator.  Similarly the CO2 and O2 gas pressures are different inside and outside of an incubator.  However, thanks to the time-lapse technology, which permits taking the pictures of embryos within the incubator every 10-15 minutes without giving any harm to the embryos.  In other words, time-lapse technology permits undisturbed culture conditions, which avoids stress to the embryos.
 
Another advantage of time-lapse technology is that it permits continuous development of the embryo, rather than just giving static information at only several time-points.  It has been clearly shown that transferring embryos with several division patterns are associated with very low pregnancy rats (direct division-division of 1 cell to 3 cells or 2 cell to 5 cells within less than 4 hours).  In other words, time-lapse technology permits us not to select those embryos (deselection).  Obviously, deselection would not be possible with classical incubators. 
 
Time-lapse technology is high technology and costly.  In our embryology lab, we have 2 time-lapse incubators (Embryoscope).  We should stress that the rest of all our incubators are modern small, bench-top incubators providing high quality in-vitro culture conditions.  Although not critical, time-lapse technology offers some advantages including;
 
·         Embryos stay stable in terms of the temperature and the level of the oxygen and carbon dioxide.
·         Following closely all development stages of embryos and having more information about abnormalities of division and embryo development.
 
·         Not to select embryos-deselection (direct division-division of 1 cell to 3 cells or 2 cell to 5 cells within less than 4 hours)
·         Thanks to additional parameters used for embryo selection to choose the most viable one associated with highest implantation potential.